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Heart failure (HF) is a significant chronic medical condition that can significantly impact a patient’s quality of life and the financial stability of a healthcare system. Healthcare organizations are strategically working to identify educational methods that may reduce the burden of HF in the adult population. Ongoing patient education led by multidisciplinary teams remains the backbone of nursing efforts with nurses as key providers of HF education. Patients are stabilized in an acute-care setting and may be transferred to a post-acute care healthcare setting for short-term rehabilitation. This transitional care period is when patient education continues to be an important component for improving patient’s understanding of self-care related to HF. Nurses’ knowledge of HF principles has not been examined in the post-acute care healthcare setting. This four-week descriptive, correlational study assessed knowledge levels of nurses regarding evidence-based HF self-management principles utilizing the Nurses’ Knowledge of Heart Failure Education Principles (NKHFP) survey. This study was designed to determine whether years of nursing experience, educational preparation, and licensure were variables impacting overall knowledge scores. The NKHFP survey was electronically distributed to a group of 125 registered nurses (RNs) and 125 licensed practical nurses (LPNs) who care for patients with HF in a post-acute care healthcare organization. A total of 45 RNs and 47 LPNs completed the electronic NKHFP survey. There was no statistical significance between licensure and knowledge scores (p > 0.05), years of nursing experience (p > 0.36), or educational preparation (p > 0.072). Registered nurses and LPNs demonstrated knowledge deficits in evidence-based practice self-management principles.